Disease. Gummy stem blight. Causes leaf spot, stem canker, black leaf and fruit rot; common name refers to the characteristic gummy exudate. Didymella bryoniae (Auersw.) Rehm [anamorph Phoma cucurbitacearum (Fr.) Sacc.] is the causative agent of gummy stem blight (GSB), a disease affecting. Gummy stem blight of cucurbits produces a variety of symptoms which are referred to as leaf spot, stem canker, vine wilt and black fruit rot. Lesions on leaves.
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However, due to the necessity of using a broad-spectrum diagnostic primer, we could not identify a suitable loop-forward primer, and the present LAMP assay only had one loop-backward primer. Cucumber gummy stem blight. Note that some taxonomists use the author citation D.
Cankers are light brown to beige to off-white, noticeably lighter in color than foliar leaf spots Fig. Leaf lesions are round didymellz triangular, particularly those at the leaf margins, or sometimes rhomboid Figs. Therefore, this assay could be useful even for amateur farmers without the need for elaborate laboratory equipment.
Eventually all infected vines will become necrotic and occasionally the plant dies due to wilting and defoliation [1,7].
In recent years, the LAMP-based assay has grown in popularity for the detection of many plant-pathogenic fungi Niessen and Vogel, ; Denschlag et al. Sometimes they are arranged in rings on the fruit or leaf surface. PDF version for printing Impact Statement. Gummy stem blight can be distinguished from cucurbit anthracnose by the larger leaf didymeola, larger lesions that encircle petioles, and crown cankers.
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be diddymella as a potential conflict of interest. Triggered by environmental conditions, flask shaped sexual fruiting bodies called perithecia form and produce ascospores, which serve as the primary source rbyoniae inoculum 1. Materials and Methods Ethics Statement Our study does not involve human specimens or tissue samples, or vertebrate animals, embryos or tissues.
PCR products were detected by electrophoresis on a 2.
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Mycosphaerella black rot of cucurbits. Due to these advantages, the LAMP method is becoming more attractive to a wider range of users. Genomic DNA of D.
Citron Citrullus lanatus var. The sporulation and inoculum density of spores were prepared based on our previous study Li et al. Mycelia were then harvested as described bryoniaf Ling et al. GSB produces lesions on the stems and leaves, and can also be spread by seeds. Morphological, pathological, and genetic differentiation of Didymella bryoniae and Phoma spp.
Bryoniad assay showed high specificity in discriminating all D.
Although this exudate gives the disease its common name, the presence of this symptom is not diagnostic; stem injuries can provoke a similar host response.
Plants and Tea, Japan This pathogen is also common in greenhouses where cucumbers are grown . Author information Article notes Copyright and License information Disclaimer.
If the tissue is not turgid, the pieces should be submersed in tap water to rehydrate for several minutes before proceeding. Content is available for non-profit, educational use under our Copyright Agreement. Pycnidia release tendrils of conidia under moist conditions in a gummy substance, and are dispersed by water splashing. The sequences used for LAMP primers are indicated by different colors and arrows. Received Jun 15; Accepted Aug A taxonomic survey of some species of Didymella and Didymella -like species.
This is to ensure that we give you the best experience possible. On watermelon leaves, the shape of anthracnose spots is distinctly angular and irregular, while leaf spots of gummy stem blight generally are round.
In general, the pathogen is found most commonly in humid, temperate, semi-tropical, and tropical regions 2, To increase the number of plants that can be inoculated in a minimum of space, three seedlings per cm-diameter pot may be used. The prospective application of this diagnostic tool for early and accurate detection of the causal agent of GSB in cucurbit crops could also lead to reduced fungicide use, thus benefiting the environment.
Fruits are penetrated through wounds and pollination flower scars . Use of loop-mediated isothermal amplification for detection of Ophiostoma clavatumthe primary blue stain fungus associated with Ips acuminatus. Muskmelon Cucumis melo L. As of 1 Januarychanges to the International Code of Botanical Nomenclature specify that each fungus is to be known by only one name, even if it has teleomorphic sexually reproducing and anamorphic asexual states. Common Names and Diseases.